A Technical Note for Improvement of the E12 Technique
J. Fasel, R. Mohler and B. LehmannSummary
Tissue slices of 2 - 3 mm uniform thickness are needed for the E12 technique. When large specimens are sawed to these dimentions, friction generated by the saw blade tends to soften the tissue and render the sections unacceptable. This paper dscribes a mean of modifying a commercially available band saw so that the tissue being sectioned is cooled with liquid nitrogen. Construction is relatively easy and inexpensive (US $200.00). We have found that using this instrument according to the procedure recommended results in completely acceptable sections.